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Hirimuthugoda NY1*, Perera PWA1, Aluthmuhandiram VJ2, Rashmi Ishuwara A3, Premakumara MMK1 and Lal PK1
1Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
2Center of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Thailand
3Department of Export Agriculture, Uva Wellassa University, Sri Lanka
Abstract
A fertile mangrove community can be seen along the sheltered coastline of Sri Lanka, which is an island in the
Indian Ocean. Currently there are 21 species of mangroves that have not yet been genetically classified.
Therefore, we intended to perform the Sri Lankan mangrove classification using the
rbcL gene marker. Cetyltrimethyl
ammonium bromide (CTAB) protocols for DNA extraction from mangroves and Silica extraction
method were optimized to extract DNA. All the sequence data obtained in this study were deposited in the NCBI
GenBank. Maximum Likelihood and Bayesian phylogenetic trees revealed that, the Sri Lankan mangrove
community can be classified genetically under 21 species and the Rhizophora anamalayana plant was identified as
a hybrid of two species, R. apiculata and R. mucranata. As far as
Ceriops is concerned, the vegetation of the
Trincomalee and Puttalam areas seems to be distinctly different. Therefore, need to do more research on this
clade in the future. The results of this study can be used as the baseline data for mangrove taxonomy in Sri Lanka
and will be supported in future conservation and management actions.
Keywords:
Mangroves, rbcL
marker, Rhizophora anamalayana , Sri Lanka
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* - Corresponding Author
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